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Speaker at  and Expo on Applied Microbiology 2023 - Mussarat Shaheen
Government College University Faisalabad, Pakistan
Title : Characterization, diversity and phylogenetic analysis of lipase producing indigenous bacteria collected from oil contaminated soil sites in Faisalabad

Abstract:

The main aim of the current study was to explore potential of indigenous bacteria for lipase production, isolated from different uninvestigated oil contaminated soil sites in Faisalabad. Microbes are considered an excellent source of industrial enzymes. These enzymes are usually more stable and environment friendly. Many bacterial enzymes like lipase, protease, amylase, phosphatase etc., have been used in different fields e.g., Bioremediation, leather industry, detergents, textiles, as additives in the food industry, starch processing, wastewater treatment, cosmetics, pharmaceuticals, biomedical assays and in DNA-technology. The present study was carried out in Research Laboratory, Department of Zoology, Government College University, Faisalabad. Total twenty-five soil samples were collected aseptically from different oil contaminated sites (workshop, oil exchange, truck stand, hotels and generator soil site). Tween20 agar plates were used for isolation and screening of lipase producing bacteria. Maximum CFUs (196×105) and lipase zones (124) around colonies were found in workshops soil samples, while largest lipase zone (4.9 mm) was also found in (W-3) workshop soil sample. On the basis of size of zone of hydrolysis, bacterial isolate SW3 was selected for further lipase studies. Maximum growth of SW3 isolate (4.541±0.06, optical density) and lipase production (11.66±0.3U/ml) was observed at pH 6 as compared to pH 7 and pH 8. 37?C was found optimum temperature for isolate growth (4.48±0.08, optical density) and enzyme production (10.58±0.53U/ml) as compared to 25?C and 45?C. Bacterial isolate SW3 growth (4.541±0.06, optical density) and lipase production was observed maximum (11.66±0.3U/ml) in the presence of olive oil as substrate in comparison of Tween20. Optimum time was 48 hours of incubation for bacterial growth as well as enzyme production. Isolate SW3 showed resistance to NaCl (0.5 to 5 % concentration)  Overall truck stand contaminated soil site was slightly more diverse in terms of lipase producing bacteria (1.54) as compared to other four contaminated sites by Shannon diversity index (H’) analysis. Results were found significant at (P<0.05) through analysis of variance (ANOVA). Bacterial isolate SW3 was identified as Ochrobactrum anthropi showing 99% sequence similarity to genus Ochrobactrum through 16S rRNA sequencing analysis. Present study is an effort to isolate and characterize indigenous lipase producing bacteria from different unexplored oil contaminated soils sites in Faisalabad.

Biography:

Ms. Shaheen is working as lecturer at GCUF, Faisalabad, Pakistan and also submitted Ph.D thesis as a Ph.D student in Department of Microbiology at Quaid-e-Azam University, Islamabad, working on metagenomic detection of bacterial enzyme genes from glaciers samples collected from glaciers of Pakistan. During Ph.D, she visited Bristol Glaciology center (University of Bristol, UK) under 6 months research fellowship program. She is also teaching at Government College University. During her master degree her research work was about “Incidence of Pseudomonas sp isolated from different clinical samples”, NIH, Islamabad. In M.Phil, she did her work related to “Studies on production, optimization, characterization and purification of hydrolytic enzymes produced by oil degrading Bacillus subtili”.

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