Title : Molecular Response Analysis for Lentinus squarrosulus AF5 under Azo dyes Stress: An Integrated Proteome Dynamics and Putative Metabolic Cascade
Abstract:
Effluents released from the textile industry contain about 10–15% of the dyes. These dyes if not treated can cause major environmental concerns. These dyes besides being highly ecotoxic may cause reduced transparency of the water and as a result, reduces penetration of solar light and photosynthesis. Mycoremediation of textile effluents using fungi is an effective and eco-friendly approach for decomposing or mineralizing synthetic dyes. In this study, Lentinus squarrosulus AF5 (MN215236.1) was isolated from local dye effluents. The strain had the remarkable ability of decolorization (50 - 96%) within 72 h using the dye mix (500 mg L-1) consisting of AB10B, RB5, RB160. Differential pattern in the protein profiles of the mycelial extract of the L. squarrosulus AF5 was observed. Observations indicated the induced oxidoreductase activity during the dye treated condition, thus denoted these to be involved in the remediation of the dyes. The other factors appear to be involved consist of increased metabolic and catalytic activities along with structural molecular constituents. Besides, it has been observed that several members chaperone family proteins (Hsp70, Hsp90) are upregulated during stressful conditions. The presence of Cytochrome P450 monooxygenases and oxidoreductases in the proteomics analysis provided additional evidence that the oxido-reduction process may be involved during degradation process. Notable titers of manganese peroxidase (258.84 ± 0.001 U ml-1) and relatively lower levels of lignin peroxidase (194.98 ± 0.002 U ml-1) and laccase (134.33 ± 0.007 U ml-1) in L. squarrosulus AF5 supernatants were observed. The UV-Visible, FTIR spectroscopic along with HPLC and LC-QTOF-MS analyses of the dye supernatants following incubation with L. squarrosulus AF5 had indicated the degradation of the dyes. Phytotoxicity assay with respect to the plant Wheat (Triticum aestivum) and Guar (Cyamopsis tetragonoloba) revealed that metabolites produced following degradation of dyes denoted their non-toxicity for germination of the seeds for the selected plants. Hence, L. squarrosulus AF5 appear to be a potential strain for azo dyes degradation. Furthermore, this study may also enable in developing an effective strategy for the bioremediation of the azo dyes.
