Title : Rapid Method for Detecting Pathogenic Microorganisms in Food
Abstract:
Reference methods for the detection of pathogenic microorganisms Salmonella spp. in accordance with PN-EN ISO 6579-1: 2017-04 or Listeria monocytogenes and Listeria spp. in accordance with PN-EN ISO 11290-1: 2017-07 are labour-intensive and time-consuming. These classic methods for detecting Salmonella spp. in food takes 3 to 5 days, while for detecting Listeria monocytogenes in food takes 5 to 7 days. Alternative methods for the shortest possible detection time of these microorganisms are constantly being sought in order to be able to release food products from the processing plant as quickly as possible. One of such methods is LAMP (Molecular Detection System 3M) method. This method uses Loop-mediated Isothermal Amplification system to rapidly amplify nucleic acid sequences with high specificity and sensitivity, combined with bioluminescence to detect amplification. Positive results are reported in real time, negative results are displayed after the test is completed. Positive results must be confirmed with a reference method. The analysis with this method takes 22-26 hours depending on the tested microorganism. The Microbiological Research Laboratory of the Dairy Industry Innovation Institute has validated and accredited the Salmonella spp., Listeria monocytogenes and Listeria spp. detection method using the LAMP molecular method in environmental samples from the area of ??food processing, distribution and marketing in different dairy products. During the validation tests, the laboratory analysed 364 samples for Salmonella spp., 360 samples for Listeria monocytogenes and 360 samples for Listeria spp. The validation process revealed the equivalence of the alternative method to the reference method.
The RT-LAMP (reverse transcription loop-mediated isothermal amplification) method of nucleic acid amplification can be also used to detect the RNA of the SARS-CoV-2 coronavirus in food production and distribution environment. The Microbiological Research Laboratory of the Institute of Dairy Industry Innovation has accredited this method for the detection of SARS-CoV-2 coronavirus RNA in environmental samples from the area of ??food production and distribution, in environmental samples from the surface of packaging and in dairy samples such as UHT milk, powdered products, cheese.
What will audience learn from your presentation?
A new method for detection of pathogenic microorganisms in food, a comparison of new and reference methods, use the RF-LAMP method to detect SARS-CoV-2 virus in food production environment.
To widen area of their expertise and possibly to apply new method.
· How will this help the audience in their job? Is this research that other faculty could use to expand their research or teaching? Does this provide a practical solution to a problem that could simplify or make a designer’s job more efficient? Will it improve the accuracy of a design, or provide new information to assist in a design problem? List all other benefits. All above mentioned benefits.
