Title : The effectiveness of B cell and T cell epitopes cocktail as a potential vaccine against Staphylococcus aureus in two murine models
Abstract:
Development of an effective vaccine against Staphylococcus aureus (S. aureus) is a key global health concern, especially in light of rising antibiotic resistance and the wide spread of methicillin resistant Staphylococcus aureus (MRSA) strains. Previous attempts for S. aureus vaccine development were unsuccessful. Inclusion of T cell immunity in S. aureus vaccine may be essential for the effectiveness of the vaccine. Previous work in our laboratory showed that Phosphatidylinositol phosphodiesterase (PIc) B cell & T cell peptides induced high protection in mice bacteremia model. In this study, Manganese transport protein C (MTC) B cell & T cell epitopes, Nickel ABC transported (NABC) B cell & T cell epitopes and PIc B cell & T cell epitopes were used as vaccine in two mice models; bacteremia and skin infection models. Results shown robust humoral immunity in both models that was correlated with high survival level in mice immunized with PIc and peptide mixture groups in bacteremia model. In skin infection model, mice immunized with peptide mixture and MTC peptide group showed the best skin lesion healing results. The protection level of both models was correlated with the highest level of INF γ and lowest levels of IL-2 which was shown in the peptide mixture group. The mixture group also showed the highest count of CD8 cells in both models. results demonstrated that inclusion of multiple genes B cell and T cells epitopes improved both the humoral and cellular immunity and resulted in the best outcome in both bacteremia and skin infection mice models. A more expanded in-vivo study is recommended for testing MTC and PI B cells and T cells peptides cocktail as promising S. aureus vaccine.
What will audience learn from your presentation?
- Current situation regarding Staphylococcus aureus vaccine
- Role of the two arms of immunity in infection protection and vaccine efficiently
- Peptide vaccines as a new approach in vaccinology
