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Speaker at Applied Microbiology 2022 - Joanna Stec
Medical University of Lublin, Poland
Title : Detection of topoisomerase IV encoding parC gene in recreational water isolates of Aeromonas veronii

Abstract:

Quinolones are frequently used drugs owing to their characteristics of easy to use, broad-spectrum activity, and high efficiency. Their presence in the environment can pose a threat to the ecosystem and to human health. Those antibiotics can enter water bacteria through e.g. humans and aquaculture. As a consequence, they cause selection and stimulate bacterial resistance.
The aim of this study was to determine the prevalence of specific quinolone parC resistance genes in Aeromonas veronii isolated from natural water reservoir in the south-eastern Poland. This enzyme is involved with the decatenation of the interlinked daughter chromosomes. Quinolone resistance is essentialy due to chromosomal mutations.
Sixty-seven A. veronii isolates obtained from freshwater reservoir were identified on the basis of their protein profile. Antimicrobial resistance was determined by the automatic method with Vitek 2 Compact System. The parC genes was investigated by PCR.
All of the A. veronii isolates were phenotypically sensitive to levofloxacin and ciprofloxacin on the basis of MIC (Minimal Inhibitory Concentration) values. The presence of parC gene was found in 55.2% (37/67) of tested isolates.
Our study confirms the presence of quinolone resistance gene - parC in the environmental A. veronii isolates derived from the recreational water reservoir. This may facilitate fluoroquinolone resistance, and increase the risk of Aeromonas spp. infections, and potentially serve as reservoirs for the dissemination of parC genes to other aquatic microbes.

 

What will audience learn from your presentation?

  • The information helps to understand the possible risk of infections by the use of recreational waters by people (especially by those immunocompromised) as Aeromonas veronii cause for example skin and soft-tissue infections, wound infections, urinary tract infections.
  • The presence of quinolones in the environment can pose a threat to the ecosystem and to human health. Those antibiotics can enter water bacteria through e.g. humans and aquaculture. As a consequence, they cause selection and stimulate bacterial resistance. This work increase the knowledge on prevalence of the quinolones resistance-gene parC in recreational waters microbes;

Published data pay attention to potential reservoirs for the dissemination of parC genes to other aquatic microbes. Such information can contribute to design of other scientific studies and provide additive information to complete other research

Biography:

Joanna Stec studied Biotechnology at the University of Agriculture in Cracov and graduated as Master of Science in 2015. Then, in 2017, she graduated from postgraduate studies in Medical Analyst obtaining the professional title of laboratory diagnostician. In 2018 she joined the Chair and Department of Pharmaceutical Microbiology in the Medical University of Lublin to start her PhD

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